. 2016 Jun 17;11(6):e0157758. doi: 10.1371/journal.pone.0157758

Table 1. Summary of hCDKL5 transcript isoforms.

Human
		X-chromosome coordinates		CDKL5 isoform
Exon	Size (bp)	Exon start	Exon end	1					2	3	4	5
1	88	18425608	18425695	+	+				+	+	+
1a^#	333	18426069	18426401			+
1b^#	229	18426691	18426919				+
	44	18426876	18426919		+
1c	38	18442224	18442261									+
1d	49	18442375	18442423									+
1cd^#^$	200	18442224	18442423										+
1e^#	121	18443839	18443959					+						+
	141	18443819	18443959										+
2	226	18506935	18507160	+	+	+	+	+	+	+	+	+	+	+
3	35	18510820	18510854	+	+	+	+	+	+	+	+	+	+	+
4	46	18564477	18564522	+	+	+	+	+	+	+	+	+	+	+
5	137	18575354	18575490	+	+	+	+	+	+	+	+	+	+	+
6	121	18579848	18579968	+	+	+	+	+	+	+	+	+	+	+
7	60	18581891	18581950	+	+	+	+	+	+	+	+	+	+	+
8	91	18584263	18584353	+	+	+	+	+	+	+	+	+	+	+
9	190	18587954	18588143	+	+	+	+	+	+	+	+	+	+	+
10	81	18595348	18595428	+	+	+	+	+	+	+	+	+	+	+
11^#	152	18598462	18598613	+	+	+	+	+	+			+	+	+
	101	18598462	18598562							+	+
12	967	18603902	18604868	+	+	+	+	+	+	+	+	+	+	+
13	102	18608811	18608912	+	+	+	+	+	+	+	+	+	+	+
14	106	18609465	18609570	+	+	+	+	+	+	+	+	+	+	+
15	124	18613152	18613275	+	+	+	+	+	+	+	+	+	+	+
16	100	18619867	18619966	+	+	+	+	+	+	+	+	+	+	+
17	123	18623879	18624001						+		+
18	120	18625128	18625247	+	+	+	+	+	+	+	+	+	+	+
19^#	217	18628371	18628587									+	+	+
	7025	18628371	18635395	+	+	+	+	+	+	+	+
	10037	18628371	18638408	+_XL	+_XL	+_XL	+_XL	+_XL	+_XL	+_XL	+_XL
20	84	18646007	18646090									+	+	+
21	183	18650410	18650592									+	+	+
22^#	199	18653432	18653630									+	+	+

^# novel exon, splice site or UTR, not previously characterised

^$ exon 1cd represents a readthrough of exon 1c to the end of exon 1d

Exon numbers, sizes and co-ordinates for the human CDKL5 gene (GRCh38/hg38 assembly). Exon composition of each transcript isoform is indicated. Starting coordinates for the main TSS of each initial exon are italicised; coordinates of polyadenylation sites in each terminal exon are underlined. Bold indicate exons that contain multiple splice sites. Note that isoform hCDKL5_1 is labelled as having sub-isoforms beginning at each of the alternative first exons, 1a, 1b and 1e; this is also expected to be true for isoforms hCDKL5_2, _3 and _4. A subscripted ‘XL’ symbol signifies usage of a more distal polyadenylation signal and consequently the presence of an extended 3’-UTR.