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. 2016 Jun 17;12(6):e1006117. doi: 10.1371/journal.pgen.1006117

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© 2016 Laffitte et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A, D) L. infantum WT clone (lane +), (B, E) L. infantum MRE11^-/- clone (lane +) and (C, F) L. infantum MRE11^-/-RAD50^-/- clones (lanes 1–10) were selected for MTX resistance up to 1600nM MTX, their chromosomes were separated by pulsed-field gel electrophoresis using a separation range between 150kb and 1500kb, transferred on membranes then hybridized with PTR1 (A, B, C) and DHFR-TS (D, E, F) probes. Lanes 0 and lanes—are parasites without drug selection.