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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Int J Parasitol. 2015 Jul 14;45(12):773–781. doi: 10.1016/j.ijpara.2015.06.001

Fig. 4.

Fig. 4

Endocytosis pathway inhibitors block the uptake of Opisthorchis viverrini excretory/secretory products (OvES) by normal (H69) and cholangiocarcinoma (KKU-100 and KKU-M156) biliary cell lines using flow cytometry. Cells were pretreated with the endocytosis inhibitors chlorpromazine (CPZ), sucrose (clathrin-mediated inhibitor) and filipin (caveolae-mediated inhibitor) before incubation with OvES for 2 h. Fluorescence intensity was measured by flow cytometry (A). The histogram depicts the average of three independent experiments ± S.E.M. (B).