Table 6.
Selected qRT-PCR determinations of fold changes in gene expression in 1,600 μg/mL 3.5 nm MPA-capped CdTe QD treatment, compared with RNA-seq data
| Gene symbol | qRT-PCR
|
RNA-seq
|
Description | ||
|---|---|---|---|---|---|
| Fold change | P-value | Fold change | Corrected P-value | ||
| GNGT2 | 12.78 | 9.61−8 | 46.28 | 1.68−8 | Guanine nucleotide-binding protein (G protein), γ-transducing activity polypeptide 2 |
| P2RX1 | 3.28 | 6.54−8 | 20.95 | 5.65−3 | Purinergic receptor P2X, ligand-gated ion channel 1 |
| ADCY7 | 4.32 | 4.33−7 | 9.39 | 1.17−5 | Adenylate cyclase 7 |
| PRKCD | 4.64 | 4.44−8 | 6.80 | 1.47−5 | Protein kinase Cδ |
| ARHGDIB | 5.86 | 1.92−6 | 5.81 | 5.10−5 | Rho, GDP-dissociation inhibitor (GDI) beta |
| ADCY4 | 3.67 | 2.75−6 | 4.92 | 1.38−3 | Adenylate cyclase 4 |
| DUSP2 | 2.27 | 1.70−3 | 4.78 | 8.27−3 | Dual-specificity phosphatase 2 |
| NFKB2 | 2.62 | 3.97−5 | 4.30 | 6.49−3 | Nuclear factor of κ-light polypeptide gene enhancer in B cells 2, p49/p100 |
| GNA15 | 3.03 | 1.18−3 | 4.27 | 3.63−3 | Guanine nucleotide-binding protein, α15 |
| PIK3R5 | 3.18 | 9.98−7 | 4.20 | 4.18−3 | Phosphoinositide-3-kinase, regulatory subunit 5 |
| PTPN7 | 3.13 | 3.33−3 | 3.94 | 1.27−2 | Protein tyrosine phosphatase, nonreceptor type 7 |
| SHC1 | 1.73 | 3.23−3 | 3.42 | 1.08−2 | SHC (Src homology 2 domain-containing) transforming protein 1 |
| PTAFR | 2.13 | 3.78−5 | 3.41 | 1.22−2 | Platelet-activating factor receptor |
| ADCY8 | 0.37 | 7.06−4 | 0.32 | 3.29−2 | Adenylate cyclase 8 (brain) |
| TRHR | 0.18 | 1.91−5 | 0.18 | 1.16−3 | Thyrotrophin-releasing hormone receptor |
Abbreviations: qRT-PCR, quantitative real-time reverse-transcription polymerase chain reaction; MPA, 3-mercaptopropionic acid; QD, quantum dot.