Figure 1.

Analytical validation of fusion gene detection by hybrid-capture-based comprehensive genomic profiling (CGP). (A): Five cell lines harboring characterized fusions were mixed into 22 variably sized pools such that each fusion was represented at 20%, 25%, 33%, 50%, and 100% cellular fraction at least once, for a total of 32 gene-fusion test cases. (B): Repeated testing of clinical formalin-fixed, paraffin-embedded (FFPE) specimens identified to harbor fusions demonstrated detection reproducibility within and across assay batches. (C): Summary of concordance data between fluorescence in situ hybridization and nonhybrid-capture-based CGP for 45 clinical FFPE specimens validated for the presence or absence of ALK rearrangements. (D): Breakpoints in ALK rearrangements detected by CGP.