Figure 1.

Differentiation of hPSCs into Cranial Placode Using Chemically Defined Conditions

(A) Schematic representation of cranial placode in vivo development and protocol for directed differentiation of hPSCs. ICM, inner cell mass.

(B) Real-time PCR gene expression time course of key cranial placode (SIX1, EYA1) and non-neural ectoderm (TFAP2A, DLX3/5, GATA3) genes as well as genes probing for potential contaminates (SOX10, T, SOX17, MYOD). Values are normalized to GAPDH and expression on day 0 of differentiation (directly before switch to differentiation medium) and plotted as mean ± SEM from four independent differentiations. Numbers above day-11 data point indicate average raw ct values for better comparison.

(C) Immunofluorescence analysis comparing protein expression on day 11 of cranial placode induction protocol and LSB (LDN-193189/SB-431542; neuroectoderm). Scale bars, 50 μm.

See also Figure S1.