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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2016 Apr 9;96:34–44. doi: 10.1016/j.freeradbiomed.2016.04.003

Figure 2. Dose- and time-dependent effects of H2O2 on NaV1.5 expression in HL-1 cardiomyocytes.

Figure 2

(A) Western blot and real-time PCR were performed to evaluate the expression of β-catenin and NaV1.5 in HL-1 cells after 0, 10 μM, 25 μM or 50 μM H2O2 treatment for 48 hours. NaV1.5 protein and mRNA levels were significantly decreased (p<0.05) with H2O2 in does-dependent manner but β-catenin expression was not significantly affected. (B) Western blot and real time-PCR were performed to evaluate the expression of β-catenin and NaV1.5 expression in HL-1 cells after 50 μM H2O2 treatment for 0, 12 hours, 24 hours or 48 hours. Western blot and real-time PCR showed that NaV1.5 protein and mRNA were significantly decreased with time-dependent manner (p<0.01 and p<0.05) after treatment but β-catenin protein was not significantly altered. *p<0.05 and ** p<0.01; n=3 batches of cells from 3 independent experiments carried on separate occasions for each group.