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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2016 Apr 9;96:34–44. doi: 10.1016/j.freeradbiomed.2016.04.003

Figure 8. Knockdown of TCF4 attenuated β-catenin inhibition of SCN5a promoter activity.

Figure 8

(A) Both β-catenin overexpression and LiCl significantly inhibited the SCN5a promoter activity (p<0.01), compared to the control groups, respectively. (B) Luciferase promoter assays revealed that β-catenin significantly inhibited SCN5a promoter activity in a concentration-dependent manner (p<0.01). (C) Knockdown of TCF4 by 100 nM SiRNA did not significantly affect the activity of SCN5a promoter, compared to the 100 nM scramble SiRNA group. (D) In the presence of β-catenin, knockdown of TCF4 by SiRNA significantly increased the SCN5a promoter activity (p<0.01), compared to the β-catenin and scramble SiRNA co-transfection group. **p<0.01; Ctl: control; n=3 batches of HeLa cells from 3 independent experiments carried on separate occasions for each group.