Figure 1. Antipsychotics and other psychiatric drugs affect zebrafish behavior.

(a) Plot showing the average motion index (MI) of control animals as a function of time. Assay names and their order in the battery are indicated as x-axis labels. The MI time series (black) is the average of 48 time-series, each obtained from a well containing 8 larvae and the shaded area (gray) covers ± 3*sem. All wells were located on the same 96-well plate. (b) Line plots showing phenotypic distance (y-axis) of the indicated compounds from DMSO control phenotypes at the indicated concentration (x-axis). The phenotypic distance of the DMSO phenotype from itself (0μM) is arbitrarily set to 0.1. The phenotype for each condition (compound & concentration) is the average of 36 time-series obtained from 36 wells screened over 3 daily experiments of 12 wells each. Each well contained 8 larvae. The phenotypic distances shown are distances between average phenotypes, so effectively n=1. Marker size represents, for each phenotype, the MI averaged over time, i.e. the area under the curve for the MI time series. Colors represent different classes of psychiatric drugs (Blue, antipsychotics; yellow, antidepressants; purple, anxiolytics). (c) Multi-dimensional scaling representation of the pairwise distances between MIs of animals treated with the indicated compounds. Larger marker sizes indicate greater concentrations. Gray circles represent equal volumes of DMSO. Each data point is the average of the same 36 time series used for panel b.