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. Author manuscript; available in PMC: 2017 Jun 16.
Published in final edited form as: Cell. 2016 May 26;165(7):1734–1748. doi: 10.1016/j.cell.2016.05.001

Figure 1.

Figure 1

Expression of Ms4a genes in necklace sensory neurons. A. (Left) The Gucy2d-IRES-TauGFP allele marks necklace sensory neurons expressing PDE2A (blue) and GC-D (red). Grayscale is nuclear counterstain (DAPI). (Right) Pde2a+ necklace sensory neurons reside in epithelial “cul-de-sacs” and do not express the Omp-IRES-GFP allele or the conventional OR signal transduction protein Adenylyl Cyclase3 (red). Scale bars 10 μM. B. Enrichment versus expression plot for every sequenced mRNA in GC-D+ and OMP+ sensory neurons. Each point is a transcript with detectable RNAseq reads, with marker genes associated with GC-D cells (Car2, Pde2a, and Cnga3) and OMP cells (Golf, Cnga2, and Cnga4) labeled in red and green, respectively; reliably detected Ms4a family members are highlighted in blue. C. Nanostring-based mRNA quantification of GC-D cells relative to OMP cells. Marker genes for OMP and GC-D cells are shown in green and red, respectively. Twelve of the 17 annotated Ms4a genes are significantly enriched in GC-D cells relative to OMP cells. * p < 0.05, unpaired t-test. See also Figure S1.