Figure 4.

Dissociated Cortical Neurons Respond to Depolarization

(A) Representative images of immunocytochemistry of HUES8-derived cortical neurons 20 days after dissociation from spheres, before and after stimulation by KCl. Cells were stained using antibodies specific for MAP2 (purple), CTIP2 (red), and phosphorylated forms of CREB (green). Scale bars represent 50 μm.

(B) Quantification of the relative proportion of neurons staining positive in immunocytochemistry experiments using an antibody specific for phospho-CREB. Error bars indicate SD from three independent experiments.

(C) Quantification of expression of NPAS4, cFOS, ARC, and BDNF by qRT-PCR, following stimulation by KCl. Error bars indicate SD from three independent experiments.

See also Table S2.