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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Cancer Res. 2016 Mar 16;14(6):563–573. doi: 10.1158/1541-7786.MCR-15-0450

Figure 1. PTK6 promotes the epithelial phenotype in human colon cancer cell lines.

Figure 1

Relative expression of PTK6, the epithelial markers E-Cadherin and ZO-1, and the mesenchymal markers N-Cadherin, ZEB-1, Claudin-1 and Vimentin was examined in SW480 and SW620 colon cancer cell lines, and SW480 cells stably expressing shRNA constructs targeting PTK6, shRNA49 and shRNA52, or a non-targeted shRNA control, shSCR. Immunoblotting was performed using total cell lysates from preconfluent cultures. (A) SW480 and SW620 parental cell lines and (B) SW480 PTK6 knockdown cell lines. Expression of RNAs encoding EMT markers was analyzed using qRT-PCR (CDH1 = E-Cadherin, SNAI1 = Snail, SNAI2 = Slug). (C) parental SW480 and SW620 cell lines and (D) SW480 PTK6 knockdown cell lines (*p<0.05 **p<0.005). (E) Immunofluorescent staining for protein expression and localization of E-Cadherin and ZEB-1 in SW480 PTK6 control and knockdown cells is shown (size bar = 100 μm).