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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Acta Neuropathol. 2016 Apr 8;132(1):59–75. doi: 10.1007/s00401-016-1572-y

Fig. 4.

Fig. 4

Benztropine restores myelin basic protein (Mbp) expression in α-synuclein-overexpressing primary oligodendrocyte progenitor cells (OPCs). a The dose-dependent effect of benztropine on Mbp expression in primary OPCs is illustrated using Western blot. Gapdh signal served as control. b Benztropine significantly increased Mbp gene expression in a range of 0.1-1.0 μM as quantified by real time PCR (n = 3-6). c Immunocytochemistry for Mbp and Olig2 illustrates the effect of 0.5 μM benztropine (BT) on OPC maturation in comparison to vehicle- (veh) treated cells. Scale bar: 20μm. d The restoration of Mbp expression in α-synuclein-overexpressing cells (EF1a-SYN-IG +veh) upon treatment with 0.5 μM benztropine (EF1a-SYN-IG +BT) to control levels (EF1a-IG +veh) is demonstrated by Western blot. Gapdh signal served as control. e Benztropine restored Mbp gene expression in α-synuclein-overexpressing OPCs as quantified by real time PCR (n = 3-6). f α-Synuclein-overexpressing cells exhibited the typical morphology of OPCs (bi-/tripolarity), whereas treatment with benztropine induced Mbp expression and formation of multiple processes as signs of advanced maturity comparable to control condition. Scale bar: 20μm. Data are shown as mean ± standard error of mean. ANOVA: *p < 0.05, ***p < 0.001. T-test: ##p < 0.01.