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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Acta Neuropathol. 2016 Apr 8;132(1):59–75. doi: 10.1007/s00401-016-1572-y

Fig. 5.

Fig. 5

Restored myelinogenic capacity of α-synuclein-overexpressing primary rat oligodendrocyte progenitor cells (OPCs) by benztropine. a A Venn diagram depicts differentially regulated genes revealed by RNA sequencing of primary rat OPCs (n = 3). Green circle: α-synuclein-overexpressing (SYN) versus control cells (CTRL). Blue circle: benztropine (BT) versus vehicle (veh)-treated α-synuclein-overexpressing cells. b Genes differentially regulated by both α-synuclein overexpression and benztropine treatment classified into gene ontologies (GOs) implicated among others in myelin formation (black), membranogenesis (dark gray), and maintenance of oligodendrocyte immaturity (light gray). c Expression dynamics of representative genes identified by GO term enrichment are listed. Benztropine (blue) oppositely regulated genes differentially expressed upon α-synuclein overexpression (green). Myelin formation: Mbp = myelin basic protein, Plp1 = proteolipid protein 1, Lpar1 = lysophosphatidic acid receptor 1, Aspa = aspartoacylase, Atrn = attractin, Tspn2 = tetraspanin 2; Membranogenesis: Hmgcr = 3-hydroxy-3-methylglutaryl-CoA reductase, Dhcr24 = 24-dehydrocholesterol reductase, Ank3 = ankyrin 3, Gsn = gelsolin, Ebp = emopamil binding protein (sterol isomerase), Scd1 = stearoyl-CoA desaturase-1; Maintenance of immaturity: Sox9 = sex determining region Y-box 9, Tcf7l1 = transcription factor 7-like 1, Nkx2.2 = Nk2 homeobox 2, Mt3 = metallothionein 3, Hey1 = hes-related family bHLH transcription factor with YRPW motif 1, Cst3 = cystatin C.