Figure 3. Activation of CCK^NTS or DBH^NTS neurons suppresses food intake.

(a–c) Chemogenetic activation of CCK^NTS neurons decreases baseline food intake at 2 h (a) and 4 h (b) after lights out, but has no significant effect on 24-h food intake (c). (e–g) Chemogenetic activation of DBH^NTS neurons decreases baseline food intake at 2h (e) and 4 h (f) after lights out during all 4 days of CNO injection, but only effects 24-h food intake (g) on the first day of CNO injection. (d,h) Activation of CCK^NTS (d) or DBH^NTS (h) neurons decreases body weight. (i), In food restricted animals, activation of CCK^NTS or DBH^NTS neurons decreases food consumed over 1 h during the day compared to saline injection or CNO injection into mice expressing only mCherry. Data were analysed using two-way repeated measures ANOVA, bonferroni post hoc comparison, and represent mean (CCK: n=6 mCherry, 9 hM3Dq, DBH: n=6 per group)±s.e.m., *P<0.05, **P<0.01, ***P<0.001.