Table 2. Percentile ranking of Spearman correlation among 1,082 genes.

Gene mutation	MSI-H	Distant-Mets	Gene mutation				Gene signature
			APC	TP53	KRAS	BRAF	EMT	RAS
APC	0.1	99.6	—	100	100	0.1	23	0.2
TP53	0.2	99.4	99.9	—	6	0.4	74	0.1
KRAS	0.3	100	100	18	—	0.2	2	100
SMAD4	4	95	84	85	93	52	13	89
TCF7L2	22	58	98	67	70	8	49	29
FAM123B	36	13	97	31	97	22	71	80
GNAS	49	69	60	67	86	71	28	98
ADAMTS18	51	51	40	56	69	50	38	56
LRP1B	66	81	45	91	38	80	55	58
PIK3CA	67	28	85	7	98	32	1.4	51
TAF1L	81	48	39	80	21	93	24	84
FBXW7	86	12	63	56	38	78	41	54
SYNE1	93	21	4	30	50	95	24	99
CSMD3	93	34	7	48	62	99	6	99.4
CBX4	95	7	3	43	2	98	56	50
ITGB4	99.9	17	0.8	11	46	99.6	43	99.7
BRAF	100	3	0.1	16	0.1	—	37	99.9

Only genes with Z5 non-silent mutations were analysed. APC, Adenomatous polyposis coli; EMT, epithelial–mesenchymal transition; Mets, metastasis; MSI, microsatellite instability.

(1) Closer examination of the literature and our own data suggest that only the BRAF(V600E) (comprising 53/60 patients with BRAF mutations) and APC-truncating mutations (stopgains or frameshift mutations, as these mutations clearly inactivate downstream β-catenin-binding sites) should be considered as clearly functional for these two genes; hereafter, we include only BRAF(V600E) and APC-truncating mutations in the further analysis and simply refer to these specific mutations as BRAF and APC mutations.

(2) MSI-H is a binary variable for the MSI-H status.

(3) EMT and RAS refer to published gene signature scores¹⁶,¹⁷.

(4) Percentiles ≤5 or ⩾95 are shown in bold.