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. 2016 Jun 15;7:11769. doi: 10.1038/ncomms11769

Figure 1. CRISPR/Cas9 deletions in the melanin pigmentation gene Ddc result in mosaic depigmentation phenotypes in V. cardui.

Figure 1

(a) Location of sgRNAs relative to the V. cardui Ddc locus. Sequences of selected deletion alleles from eggs and the butterflies shown (M2, M4 and M9) confirm disruption of Ddc. Blue: sgRNA targets. Red: PAM sequences. (b) Ddc deletions result in depigmentation of larvae, including bilateral mosaics. (c) An example of mosaic melanin-specific depigmentation in adult wings.