Figure 3. CRISPR/Cas9 Dll deletions result in expansion and gain of eyespot colour patterns.

(a) Location of sgRNAs relative to the V. cardui Dll locus. Sequences of Dll alleles from the animals shown confirm lesions in target regions. Blue: sgRNA targets. Red: PAM sequences. Green: novel sequences not observed in wild-type alleles. (b) Dll deletion causes multiple effects on distal colour patterns of the V. cardui forewing, including an increase in size and number of eyespots. Both wings shown are from the same individual (Vc M15), where the ‘normal' wing shows a wild-type phenotype, while the ‘aberrant' wing shows several abnormalities as annotated to the right. (c) Effects of Dll mutation on the V. cardui hindwing. (d) A J. coenia Dll mutant (Jc M3) shows distal elongation of a hindwing eyespot. The white dotted circle shows the size of the wild-type eyespot on the opposing wing of the same individual. (e) J. coenia Dll deletion result in patches of dark pigmentation on both the forewing (left) and hindwing (right). The individual on the left also shows disruption of margin patterns and anterior eyespot size asymmetry.