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. 2016 Jun 18;16:113. doi: 10.1186/s12866-016-0728-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — A combination of calcineurin inhibitor CsA and FLC down-regulated the expression of biofilm-related and drug transporter in clinical C. albicans isolates. Clinical C. albicans isolates were cultured in 6-well plates with 32 μg/mL FLC and 75 μg/mL of CsA alone or in combination for 24 h. The expression of indicated genes was determined by a qRT-PCR assay. The combination of CsA and FLC significantly inhibited the expression of all tested genes, although CsA or FLC along also could moderately down-regulated the expression of these genes. Compared with the untreated group, FLC alone group, a: p < 0.05; compared with the CsA or FLC alone group, b: p < 0.01. Data represented the mean ± SD from three independent triplicated experiments (N = 9)