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. 2016 Jun 18;35:97. doi: 10.1186/s13046-016-0370-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The E545K mutation of PIK3CA promotes GBC progression in vitro. a, b GBC-SD and NOZ cells treated with CON, WT and E545K were evaluated using CCK8 cell viability assays. Cell proliferation was represented as cell quantity. Data are presented as mean ± SD (n = 5). c, d Migration assays of the GBC-SD and NOZ cells treated with CON, WT and E545K were evaluated with uncoated transwells. Representative images of the stained migrated cells stained pictures and the numbers of migrated cells (mean ± SD, n = 5) are shown. e, f Invasion assays of the GBC-SD and NOZ cells treated with CON, WT and E545K were evaluated with Matrigel-coated transwells. Representative images of stained invaded cells and the numbers of invaded cells (mean ± SD, n = 5) are shown