Fig. 5.

Localization of RMRP RNA in failing hearts. Sections were derived from FFPE LV biopsies of HF patients and RMRP RNA was detected by in situ hybridization in blue, using bright field microscopy (a, c). As negative control, hybridization was also carried out with a probe for an exogenous RNA, DapB of B. subtilis (d, f). Nuclei were detected by HOECHST 3332 counterstaining using fluorescence microscopy (b, e). The merge of RMRP and HOECHST 3332 signals c shows that RMRP RNA has a ubiquitous, mostly nuclear distribution. Representative pictures are shown (n = 10; calibration bar = 20 µm; magnification 40×)