Fig. 2.

Overview of cultivation systems with integrated substrate supply. a Multilayer system by [17, 21]. A medium-agar was covered by a broth in which the bacteria were cultivated. b Feed Bead^® System by Jeude et al. [19]. Silicone elastomer discs (feed beads) releasing substrate to the medium. c Principle of the integrated substrate delivery system with the EnBase technology [11]. Dissolved polysaccharides being part of the cultivation medium are degraded to glucose molecules by the addition of defined enzyme concentration