Table 1.
Overview of produced target molecules with advanced growth system
| Target group | Specific target | References |
|---|---|---|
| Enzymes | Monomeric variants of Trypanosoma brucei triosephosphate isomerase(TIM) | [21] |
| Drosophila melanogaster multifunctional enzyme type 2 (MFE2) | [11] | |
| A-domain of human protein disulphide isomerase (PDI) | [11] | |
| Lactobacillus alcohol dehydrogenase (ADH) | [11] | |
| Tryptophan synthase complex | [52] | |
| Formate dehydrogenase (FDH) from Candida boidinii | [93] | |
| Human NSUN4, 5-methylcytosine RNA methyltransferase | [34] | |
| SdrE protein—surface adhesin of Staphylococcus aureus | [35] | |
| Zmp1—zinc-dependent metalloprotease | [36] | |
| Human lipoxygenases hALOX15 | [38] | |
| Esterase, Est 22; Axe A and Axe B | [44, 54] | |
| Rhizopus oryzae lipase (ROL) | [84] | |
| Purine nucleoside phosphorylases (PNPs) | [26, 103–105] | |
| Alkaline phosphatase (PhoA) and a phytase, AppA | [58] | |
| Surface lipoprotein SitA from Staphylococcus pseudointermedius | [42] | |
| Endopolygalacturonase from Aspergillus niger | [32] | |
| Chloramphenicol acetyltransferase I (CATI) | [45] | |
| Alkane monooxygenase (AlkB) | [43] | |
| Pseudomonas aeruginosa lipoxygenase (PA-LOX) | [39] | |
| Sortases: SrtC1-2b, Srt2-2b, SrtA | [46] | |
| Nitrilases | [51] | |
| Rat lactate dehydrogenase | [53] | |
| Zebrafish ALOX2 (lipoxygenase) | [40] | |
| Zinc‑dependent metalloprotease Zmp1 | [101] | |
| α-amylase AmyM from Geobacillus stearothermophilus | [88] | |
| Rhizopus oryzae lipase (ROL) in P. pastoris | [84] | |
| Antibodies, antibody fragments, other binding proteins | Two single-domain antibodies (sdAbs) | [68] |
| Four Fab fragments | [24] | |
| Anti-interleukin 1ß scFv | [58] | |
| Zinc transporter 8 autoantibodies (ZnT8A) | [49] | |
| Different nanobody variants | [60] | |
| 10 scFv and 10 Fab fragments | [70] | |
| DNA binding protein LiaR | [41] | |
| DNA binding transcriptional regulator NadR (Neisseria adhesin A regulator) | [100] | |
| RNA-binding proteins: nucleolin EF-hand domain-containing protein D2 (EFHD2), splicing factor U2AF (U2AF653) serine/arginine-rich splicing factor 1 (SRSF1) | [47] | |
| Fully reduced active RNase inhibitor: contains leucine-rich repeat motifs (LRR) | [22, 48, 98] | |
| Disulfide bonded proteinsa | Reteplase, a fragment of tissue plasminogen activator which contains only the kringle 2 and protease domains (nine disulfide bonds) | [7] |
| Chimeric-truncated form of tissue-type plasminogen activator (t-PA) | [33] | |
| Human leukemia inhibitory factor (hLIF)—a cytokine (three intramolecular disulfide bonds) | [50] | |
| AppA, a phytase (four disulfide bonds) | [58] | |
| Human growth hormone hGH | [58] | |
| Human-derived glycosyltransferase GalNAcT2 (five disulfide bonds) | [59] | |
| Bioactive peptides | Super-large non-ribosomal peptide synthetase valinomycin synthetase subunits Vlm1 (370 kDa) and Vlm2 (284 kDa) | [72] |
| Nonribosomal peptide valinomycin | [73, 75, 106] | |
| Polyketide macrolide 6-deoxyerythronolide B (6dEB) in Bacillus subtilis | [76] | |
| Ribosomal peptide microviridin | [71] | |
| Others | Plasmid DNA | [77–79] |
| Capripoxvirus proteins 64 and 95 | [25] | |
| Fusion protein: multiple-epitope antigen CTB-UE | [69] | |
| Saporin L3 from Saponaria officinalis in Pichia pastoris | [81] | |
| Human immunoglobulin receptors in P. pastoris (extracellularly) | [83] |
aAntibodies and antibody fragments are mentioned in a separate section