Fig. 2.

Multiple sequence alignment showing the conservation of wild type residues in respect to identified missense changes in FZD4 protein, TSPAN12, and ZNF408 across various species. Except p.M232V of ZNF408 remaining all the missense mutations were highly conserved across different species. Reference sequences of the FZD4 orthologues: Homo sapiens; AAR23924.1, Macaca mulatta; NP_001253804.1, Rattus norvegicus; NP_072145.1, Mus musculus; NP_032081.3, Gallus gallus; NP_989430.1, Xenopus laevis; NP_001083922.1, Canis lupus familiaris; XP_005633439.1, Equus caballus; XP_001489854.1, Elephantulus edwardii; XP_006895316.1, Alligator mississippiensis; XP_006261662.1, Bos taurus; NP_001193198.1, Lipotes vexillifer; XP_007448903.1, Sus scrofa; XP_005667267.1. Reference sequences of the ZNF408 orthologues: Homo sapiens; NP_079017.1, Macaca mulatta; XP_001111210.1, Felis catus; XP_003993266.1, Canis lupus familiaris; XP_003639743.3, Equus caballus; XP_001490832.1, Pantroglodytes; JAA35243.1 Bos taurus; NP_001180086.1, Callithrix jacchus; XP_009006283.1, Condylura cristata; XP_004683622.1, Sus scrofa; XP_013849819.1, Elephantulus edwardii; XP_006896777.1