Table 4.
Clone sequences of cel5 and cel48 obtained from industrial-scale biogas processes
| Clone | T-RFs (bp) | Most closely related microorganism | Identity (%) | Accession number |
|---|---|---|---|---|
| Cel5 | ||||
| OTU01 | 56 | Ruminococcus callidus | 84.8 | WP_021681794 |
| OTU02a | 85 | Echinicola vietnamensis | 58.0 | WP_015264998 |
| OTU03b | 85 | Echinicola vietnamensis | 61.4 | WP_015264998 |
| OTU04c | 106 | Flavobacterium sp. | 66.3 | WP_007808671 |
| OTU05 | 136 | Niastella koreensis | 73.7 | AEV98714 |
| OTU06d | 211 | Marinilabilia salmonicolor | 66.3 | WP_036163195 |
| OTU07e | 275 | Mahella australiensis | 57.4 | AEE96311 |
| OTU08f | 362 | Mahella australiensis | 58.4 | AEE96311 |
| OTU09 | 375 | Clostridium sp. | 72.1 | WP_033166154 |
| OTU10 | 396 | Clostridium cellulolyticum | 100 | WP_015924614 |
| Cel48 | ||||
| OTU01 | 68 | Clostridium stercorarium DSM 8532 | 57.9 | AGI39871 |
| OTU02 | 68 | Clostridium sp. Iso6-17a | 50.0 | ADM52292 |
| OTU03 | 177 | Ruminococcus sp. CAG:254 | 97.5 | CCZ84184 |
| OTU04g | 205 | Clostridium sp. Iso6-24 | 79.0 | ADM52293 |
| OTU05 | 238 | Ruminococcus sp. HUN007 | 83.2 | WP_049962845 |
| OTU06 | 238 | Streptomyces griseorubens | 61.0 | WP_037642616 |
| OTU07h | 247 | Acetivibrio cellulolyticus | 71.2 | WP_010681059 |
| OTU08 | 290 | Clostridium acetobutylicum | 50.5 | WP_010964229 |
| OTU09i | 321 | Ruminiclostridium thermocellum | 75.0 | ACT46162 |
| OTU10j | 328 | Clostridium termitidis CT1112 | 73.8 | EMS73539 |
| OTU11k | 358 | Clostridium straminisolvens JCM 21531 | 78.8 | GAE90081 |
aMost closely related to uncultured bacterium AGW24153 (identity: 100 %) from laboratory-scale anaerobic reactor
bMost closely related to uncultured bacterium AGW24153 (identity: 89.1 %) from laboratory-scale anaerobic reactor
cMost closely related to uncultured bacterium ACV50344 (identity: 73.3 %) from lignocellulose-based sulphate-reducing bioreactor
dMost closely related to uncultured bacterium AGO64733 (identity: 75.0 %) from anaerobic digester sludge
eMost closely related to uncultured bacterium AEV59723 (identity: 77.0 %) from laboratory biogas digester treating rice straw
fMost closely related to uncultured bacterium AEV59723 (identity: 76.0 %) from laboratory biogas digester treating rice straw
gMost closely related to uncultured bacterium AGO64695 (identity: 99.0 %) from anaerobic digester sludge
hMost closely related to uncultured bacterium AGO64692 (identity: 75.2 %) from anaerobic digester sludge
iMost closely related to uncultured bacterium AGO64682 (identity: 85.6 %) from anaerobic digester sludge
jMost closely related to uncultured bacterium AGO64673 (identity: 99.1 %) from anaerobic digester sludge
kMost closely related to uncultured bacterium AGO64695 (identity: 99.0 %) from anaerobic digester sludge