Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 17;16:45. doi: 10.1186/s12935-016-0317-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Vaccine information and MAGEs expression in tumor cell lines. A The expression vector for the recombinant vaccine protein including MAEG1-TBHSP70 expression vector (abbr. pET-M1H), MAGE3-TBHSP70 vector (abbr. pET-M3H) and MAGE1-MAEG3-TBHSP70 (abbr. pET-MMH) expression vector. B Protein lysates were run through electrophoresis gel and stained with Coomassie. C Purified recombinant proteins were run through electrophoresis gel and transferred into PVDF membrane, and then blotted with MAGE1 and MAGE3, showing similar pattern as coomassie staining. D Purified recombinant protein were blot with MAGE1 and MAGE3 separately. E Protein level of MAGE1 and MAGE3 in three different tumor cell line including HepG2, A549 and HHCC. F mRNA transcription levels of MAGE1 and MAGE3 in HepG2, A549 and HHCC cell lines using nest-RT-PCR