Fig. 2.

The inter-region variability by morphological criteria. Four regions have been explored: hippocampus (H), frontal cortex (FC), striatum (S) and cerebellum (C) in two different conditions, the control (left column) and the LPS (right column). a Historical parameters to characterize the microglial morphology: the cell body area and the cytoplasm area defined as the cell body area associated with the cytoplasmic area of the primary ramifications in μm². b Calculated criteria extrapolated from the Acapella™ script: the complexity index (CI) and the covered environment area (CEA), in μm². Data shown are means ± SD in the control and LPS groups (n = 7 and n = 6, respectively). The scale bars equal 10 μm. ANOVA Kruskal-Wallis test was used to compare the different regions. *p < 0.05, **p < 0.01, ***p < 0.001