Table 2.
Primer | Sense Strand | Antisense Strand |
---|---|---|
CYP11B1_Exon 1 | 5’- GTTCTCCCATGACGTGATCCCTCT – 3’ | 5’- TCCAAAGGATGCAGAGTGCC – 3’ |
CYP11B1_Exon 2 | 5’ – TGGACAGGAGACACTTTGGAT – 3’ | 5’ – TCGCCGCTTACAGCAAGAAC – 3’ |
CYP11B1_Exon 3–4 | 5’ – TGGGGACAAGGAGGATGGGATAC – 3’ | 5’ – TGGTGGAGAGGGAGAAATTGGG – 3’ |
CYP11B1_Exon 4 | 5’ – CGTGGGAAGATCCAGCCTCAG – 3’ | 5’ – GGAAGGTGAGGAATCCCCGAC – 3’ |
CYP11B1_Exon 5 | 5’ – AGGAGGAGGACACTGAAGGATG – 3’ | 5’ – AGGCAGGCTTGGCATCACC – 3’ |
CYP11B1_Exon 6 | 5’ – GGCTCTGTCGTTCTCAGGGTATGC – 3’ | 5’ – GGCGTTGAAGAGGGATTCCAGAG – 3’ |
CYP11B1_Exon 7–8 CYP11B1_Exon 9 CYP11B1_IVS7_construct |
5’ – AGAGAGCACAGGAAGCCCCATC – 3’ 5’ – GTTCCCCCTTCAGCATAATCTC – 3’ 5’ - AGTCGGATCCCTTGCTGATGACGCTCTTTG - 3’ |
5’ – CAGTCCCACATTGCTCAAGC – 3’ 5’ – GCCCTCGGGAGTTCCATTT – 3’ 5’- GTACTCTAGAATGGCTCTGAAGGTGAGGAG - 3’ |