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. 2016 Jun 17;16:37. doi: 10.1186/s12902-016-0118-6

Table 2.

Sequences of oligonucleotide primers used for PCR amplification and minigene construction

Primer Sense Strand Antisense Strand
CYP11B1_Exon 1 5’- GTTCTCCCATGACGTGATCCCTCT – 3’ 5’- TCCAAAGGATGCAGAGTGCC – 3’
CYP11B1_Exon 2 5’ – TGGACAGGAGACACTTTGGAT – 3’ 5’ – TCGCCGCTTACAGCAAGAAC – 3’
CYP11B1_Exon 3–4 5’ – TGGGGACAAGGAGGATGGGATAC – 3’ 5’ – TGGTGGAGAGGGAGAAATTGGG – 3’
CYP11B1_Exon 4 5’ – CGTGGGAAGATCCAGCCTCAG – 3’ 5’ – GGAAGGTGAGGAATCCCCGAC – 3’
CYP11B1_Exon 5 5’ – AGGAGGAGGACACTGAAGGATG – 3’ 5’ – AGGCAGGCTTGGCATCACC – 3’
CYP11B1_Exon 6 5’ – GGCTCTGTCGTTCTCAGGGTATGC – 3’ 5’ – GGCGTTGAAGAGGGATTCCAGAG – 3’
CYP11B1_Exon 7–8
CYP11B1_Exon 9
CYP11B1_IVS7_construct
5’ – AGAGAGCACAGGAAGCCCCATC – 3’
5’ – GTTCCCCCTTCAGCATAATCTC – 3’
5’ - AGTCGGATCCCTTGCTGATGACGCTCTTTG - 3’
5’ – CAGTCCCACATTGCTCAAGC – 3’
5’ – GCCCTCGGGAGTTCCATTT – 3’
5’- GTACTCTAGAATGGCTCTGAAGGTGAGGAG - 3’