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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Virology. 2016 May 11;495:52–62. doi: 10.1016/j.virol.2016.05.005

Fig 1. HPV18 and EBV genomes are maintained alone and together in NOKs cells over several passages.

Fig 1

(A) The HPV18 genome is maintained in NOKs cells +/− EBV over six passages. Total genomic DNA (7 μg) isolated from three independent replicate populations (labeled as #1, #2, and #3) of NOKs cells +/− EBV harboring HPV18 wild-type genomes at passage 0 and 6 post-HPV transfection was double digested with a single cutter of the HPV18 genome (Nco1) and Dpn1 (an enzyme that cuts only bacterially-replicated input DNA). The digested DNA (2.5 μg) was separated using a 0.8% agarose gel and transferred to a Hybond N+ membrane followed by HPV18-specific Southern blot analysis. (B) The EBV genome is maintained over several passages (>15) in NOKs cells without HPV18 (left panel) and with HPV18 (right panel). The number of EBV genomes per cell was quantified in EBV-infected NOKs cells grown for 12 passages in the presence (C) or absence (D) of selection for EBV, highlighting the loss of EBV genomes in the absence of selection. The number of EBV genomes per cell was also quantified in EBV-infected NOKs cells harboring HPV18 grown for 12 passages in the presence (E) or absence (F) of selection for EBV.