Representative histology images showing the center of the lesion (top row), the lesion interface (middle row), and the surrounding liver parenchyma (bottom row) after day 0, 3, 7, 14, and 28. Initially, lesions were almost solely composed of extravasated red blood cells and fibrin on day 0 (A, F). Fibroblasts started to grow into the lesion from the interface together with inflammatory cells and foci of bile ductular proliferation at day 3 (G) and day 7 (H). At day 14 (D, I) and day 28 (E, J), the majority of the lesion was replaced by regenerated liver parenchyma with only a small focus of residual scar and foreign body giant cells with calcified undigested debris in their cytoplasm. The surrounding liver parenchyma is unremarkable with no significant inflammation, necrosis, or fibrosis (K–O). LP=liver parenchyma, HL=histotripsy lesion, BD=bile ducts, Fb=fibroblasts, IC= inflammatory cells, M=macrophages, GC=giant cells.