Figure 2. Chemotherapy is toxic when administered two days after αCD40 therapy, regardless of tumor-bearing status of the host.
(A) Mice were injected with 4662 PDA tumor cells (tumor-bearing) or left uninjected (tumor-free) on day 0, and received αCD40 on day 11 and Gem on day 13. Survival curve representative of 4 experiments, 5–10 mice/group. (B) Mice were treated as described in Fig. 1, except that tumor-bearing mice received either 100 or 300 µg of αCD40 on day 11 as indicated, followed by Gem on day 13. Survival representative of 2 experiments, n=5 mice/group. (C) Mice were treated as described in Fig. 1, except that Gem was administered 3 days or 4 days after αCD40, as indicated. Survival curve representative of 2 experiments, n=3–5 mice/group. (D) Mice were treated as described in Fig. 1, except that mice received nab-paclitaxel (nP) instead of Gem on 2, 3, or 4 days, as indicated, after αCD40 treatment, n=3 mice/group. Each symbol represents a group of mice, statistical analyses by Kaplan-Meier (* indicates P < 0.05).