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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Neuropharmacology. 2016 Mar 3;107:40–48. doi: 10.1016/j.neuropharm.2016.02.036

Fig. 1.

Fig. 1

Generation of AS-PKCε knock-in mice. (A) Schematic showing targeting strategy for generating the M486A mutation (red asterisk) in exon 11 of the mouse Prkce gene. DTA = diphtheria toxin A expression cassette for negative selection; Neo = neomycin expression cassette for positive selection. Triangles represent loxP sites for Cre-recombinase mediated excision of the Neo cassette in embryonic stem cell clones. (B) PCR of tail DNA demonstrated presence of mutant (A) and wild type (+) alleles. (C) Western blot analysis showed similar levels of PKCε immunoreactivity in AS-PKCε (A/A) and wild type (+/+) hippocampus. (D) The distribution of brain PKCε immunoreactivity was similar in AS-PKCε (A/A) and wild type (+/+) mice.