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. 2016 Jun 16;2016:4536187. doi: 10.1155/2016/4536187

Figure 2.

Figure 2

Bone marrow hematopoietic stem and progenitor cell populations, BrdU incorporation, cell cycle status, and colony-forming ability are altered by lack of AHR expression. (a) Bone marrow cells were processed for Lin− cell separation, stained for specific markers, and analyzed by flow cytometry for (a) LSK and (b) LT-HSC. Bone marrow Lin− cells were isolated from BrdU-treated mice, stained for LSK cells and anti-BrdU antibody, and analyzed for BrdU+ LSK cells (c). For cell cycle analysis, BM Lin− cells were stained with DAPI and analyzed for LSK cells in G0-G1, S, and G2/M cell cycle stage (d). Colony-forming ability of primitive progenitors was assessed in BM cells (e). Data are mean ± SD, n = 5 mice/group. ∗Values significantly different from WT control (P < 0.05).