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. 2016 Jun 20;6:63. doi: 10.3389/fcimb.2016.00063

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Copyright © 2016 Zheng, Sinha, Wayne and Winkler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of pst1 or pst1-phoU1 in a ΔphoU2 mutant does not recover growth yield or antibiotic sensitivity. (A) Representative growth curves of encapsulated parent strain (IU1781), and ΔphoU2 (IU6375), ΔphoU2 Δpst1 (IU6664), and ΔphoU2 Δpst1-phoU1 (IU6854) mutants in BHI broth. Growth yields and rates are quantitated in Table S4. (B) Cefotaxime sensitivity assays for encapsulated parent strain (IU1781), and ΔphoU2 (IU6375), ΔphoU2 Δpst1 (IU6664), and ΔphoU2 Δpst1-phoU1 (IU6854) mutants. (n ≥ 3); ^***P < 0.001. See Materials and Methods, the legend to Figure 2, and the text for additional details.