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. 2016 Jun 20;6:63. doi: 10.3389/fcimb.2016.00063

Figure 5.

Figure 5

A ΔphoU1 Δpst2-phoU2 double mutant phenocopies a ΔphoU2 single mutant for decreased growth yield and increased cefotaxime sensitivity, which are even greater in a ΔphoU1 ΔphoU2 double mutant. (A) Representative growth curves of encapsulated parent strain (IU1781) and ΔphoU2 (IU6375), ΔphoU1 (IU6377), Δpst2-phoU2 (IU6550), ΔphoU1 Δpst2-phoU2 (IU6612), and ΔphoU2 ΔphoU1 (IU6499) mutants in BHI broth. (n ≥ 3) Growth yields and rates are quantitated in Table S4. (B) Cefotaxime sensitivity assays for encapsulated parent strain (IU1781) and ΔphoU2 (IU6375), ΔphoU1 (IU6377), Δpst2-phoU2 (IU6550), ΔphoU1 Δpst2-phoU2 (IU6612), and ΔphoU2 ΔphoU1 (IU6499) mutants. (n ≥ 3); ***P < 0.001. See Materials and Methods, the legend to Figure 2, and the text for additional details.