Table 1.
Relative transcript amounts of the pst1, pst2, and pnpRS operons in regulatory mutantsa.
| Strainsb | Relative transcript amount of pstS1c | Relative transcript amount of pstS2d | Relative transcript amount of pnpRe |
|---|---|---|---|
| 1. Parent strain (IU1781) | ≡1 | ≡1 | ≡1 |
| 2. ΔphoU2 (IU6375) | +16.0 ± 1.8 (n = 4) (***)f | +1.2 ± 0.6 (n = 2) (ns) | −1.2 (n = 1) |
| 3.ΔphoU1 (IU6377) | +1.6 ± 0.4 (n = 4) (ns) | +1.3 ± 0.4 (n = 2) (ns) | +1.1 (n = 1) |
| 4. ΔphoU2//phoU2+ (IU6397) | +1.3 ± 0.3 (n = 3) (ns) | NDg | ND |
| 4. ΔpnpR (IU6379) | −4.8±0.2 (n = 2) (**) | −1.1±0.1 (n = 2) (ns) | ND |
| 6. ΔpnpS (IU6496) | −2.4±0.4 (n = 2) (**) | −1.4±0.1 (n = 2) (ns) | ND |
| 7. ΔpnpRS (IU6381) | −2.0 ± 0.6 (n = 2) (*) | −1.1±0.2 (n = 2) (ns) | ND |
| 8. ΔphoU2 ΔpnpR (IU6573) | −4.4±1.1 (n = 4) (***) | ND | ND |
| 9. ΔphoU2 ΔpnpS (IU6595) | −2.1±0.3 (n = 4) (**) | ND | ND |
| 10. ΔphoU2 ΔpnpRS (IU6575) | −2.5±0.6 (n = 5) (**) | ND | ND |
| 11. ΔphoU2 ΔphoU1 (IU6499) | +19.6 ± 1.6 (n = 5) (***) | −1.4±0.1(n = 2) (ns) | +1.0 (n = 1) |
| 12. Δpst2-phoU2 (IU6550) | +43.8 ± 4.2 (n = 4) (***) | − | +1.1 ± 0.0 (n = 3) (ns) |
| 13. Δpst2 (IU6610) | +44.8 ± 1.5 (n = 4) (***) | − | ND |
| 14. Δpst2-phoU2 ΔphoU1 (IU6612) | +44.8 ± 5.5 (n = 3) (**) | − | ND |
| 15. Δpst1 (IU6638) | − | +1.0 (n = 1) | ND |
a
RNA preparation and qRT-PCR were performed as described in Materials and Methods.
b
Strains were markerless deletion mutants derived from encapsulated parent strain IU1781.
c
Relative pstS1 gene transcript amount was used to represent pst1 operon expression.
d
Relative pstS2 gene transcript amount was used to represent pst2 operon expression.
e
Relative pnpR gene transcript amount was used to represent pnpRS operon expression.
f
***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant. P-values were calculated by an unpaired t-test in GraphPad Prism. P-value is not available when n = 1.
g
ND, not determined.