Table 4.
Relative transcript amounts of the pst1 operon in different mutants in mCDM containing high and low Pi concentrationsa.
| Strainsb | Pi concentration in mCDM | Relative transcript amount of pstS1 operonc |
|---|---|---|
| Parent strain (IU1781) | 36.4 mM | ≡1 |
| 10 μM | 209.7 ± 37.9 (n = 6) (***)d | |
| ΔphoU2 (IU6375) | 36.4 mM | 24.1 ± 3.9 (n = 3) (***) |
| 10 μM | 201.6 ± 47.6 (n = 3) (***) | |
| ΔphoU1 (IU6377) | 36.4 mM | 1.4 ± 0.1 (n = 3) (ns) |
| 10 μM | 145.4 ± 25.3 (n = 4) (***) | |
| ΔphoU2 ΔphoU1 (IU6499) | 36.4 mM | 28.3 ± 0.5 (n = 3) (***) |
| 10 μM | 136.3 ± 12.6 (n = 3) (***) |
a
RNA preparation and qRT-PCR from strains in mCDM containing high and low Pi concentrations were performed as described in Materials and Methods.
b
Strains were markerless deletion mutants derived from encapsulated parent strain IU1781 (see Table S1).
c
Relative pstS1 gene transcript amount was used to represent pst1 operon expression.
d
***P < 0.001; ns, not significant. P-values were calculated by an unpaired t-test in GraphPad Prism.