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. 2016 Jun 20;7:953. doi: 10.3389/fmicb.2016.00953

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Copyright © 2016 Margarita, Rappelli, Dessì, Pintus, Hirt and Fiori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impact of M. hominis symbiosis on T. vaginalis haemolytic activity. Haemolytic activity exerted by M. hominis-infected T. vaginalis G3 and uninfected T. vaginalis G3 was compared by analysing hemoglobin released by RBCs through spectrophotometric analysis (reading at 546 nm absorbance). The values define hemoglobin released by RBCs upon contact with pathogens and represent average and error bars standard deviations for three independent experiments. Statistical significance was tested by Student t-test and ^* indicate significant (p < 0.05) variations compared to parasites without M. hominis.