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The pH-shock treatment (A) duration, (B) pH level, (C) residual acetate concentration, and (D) type of titrant did not affect Chlorella growth in flasks (250 mL, n = 2). Unless stated otherwise the pH treatment was conducted using hydrochloric acid (HCL) to reduce pH to 3.5 for 15 min in the presence of 0.5 g/L acetate and then inoculated aseptically into the Chlorella culture. Error bars represent 1 standard deviation.
