Figure 3.

Effects of ERRβ knockdown on mtDNA content. Cardiomyocytes were infected with adenovirus expressing either ERRβ-shRNA or Scr-shRNA. After a 48 h incubation period, cardiomyocytes were electrically stimulated to contract and DNA was extracted at the indicated hours of hypoxia/reoxygenation. Mitochondrial NADH dehydrogenase 1 (MT-ND1) was measured by real-time PCR and normalized to the nuclear gene GAPDH to correct for differences in genomic DNA. Values are the mean + SE, n=5 experiments. There were no significant differences between cardiomyocytes expressing Scr-shRNA versus ERRβ-shRNA.