Figure 4.

Effects of ERRβ knockdown on oxygen consumption rate (OCR). Cardiomyocytes were infected with adenovirus expressing either ERRβ-shRNA or Scr-shRNA. OCR measurements were obtained after 48 h of incubation. Non-infected cardiomyocytes were maintained in wells without electrodes and remained quiescent. Values are the mean + SE; n=5 experiments. Statistical comparisons between individual means were performed by ANOVA followed by the Fisher’s LSD test for comparisons between individual means. Although not indicated in the figure, electrically stimulated contraction (Stimulator: ON) significantly increased OCRs in cardiomyocytes expressing either ERRβ-shRNA or Scr-shRNA as compared to their quiescent state (Stimulator: OFF); p<0.0001.