Table 1.
Primer sets used for quantitative real-time RT-PCR
| Target RNA Sequence | Primer Set |
|---|---|
| Estrogen-related Receptor α (ERRα) | F: 5’-GGCCCTTGCCAATTCAGA-3’ |
| R: 5’-GGCCTCGTGCAGAGCTTCT-3’ | |
| Estrogen-related Receptor β (ERRβ) | F: 5’-CATGAAATGCCTGAAAGTGG-3’ |
| R: 5’-CACAGAGAGTGGTCAGGGCCT-3’ | |
| Peroxisome Proliferator-Activated Receptor γ Coactivator-1α (PGC-1α) | F: 5’-CAAGCCAAACCAACAACTTTATCTCT-3’ |
| R: 5’-CACACTTAAGGTGCGTTCAATAGTC-3’ | |
| Peroxisome Proliferator-Activated Receptor γ Coactivator-1β (PGC-1β) | F: 5’-CATCTGGGAAAAGCAAGTACGA-3’ |
| R: 5’-CCTCGAAGGTTAAGGCTGATATCA-3’ | |
| 18S rRNA | F: 5’-TATGGTTCCTTTGGTCGCTC-3’ |
| R: 5’-GGTTGGTTTTGATCTGATAAATG-3’ | |
| NADH Dehydrogenase Subunit 1 (ND1) | F: 5’-GCCATAATATGATTTATCTC-3’ |
| R: 5’-AGAAAGTTTTTTCATAGGAG-3’ | |
| Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) | F: 5’-AGGTCATCCCAGAGCTGAAC-3’ |
| R: 5’-CCTGCTTCACCACCTTCTTG-3’ |