. 2016 Jun 20;6:28347. doi: 10.1038/srep28347

Table 2. Relative levels of acetylation (ace), dimethylation (met) or phosphorylation (pho) in different histone sites in oocytes with various chromatin configurations after OH at different temperatures for different times^*.

Histone site	h	°C	NSN	IN	SN	c-NSN	c-IN
H3K14-ace	0	–	1.00 ± 0.00^a	1.07 ± 0.04^a	1.33 ± 0.01^a	–	–
	1	37	1.05 ± 0.01^b	1.21 ± 0.02^b	1.49 ± 0.02^b	–	–
		39	–	–	1.23 ± 0.01^c	1.00 ± 0.01^a	1.05 ± 0.01^a
	2	37	–	–	1.13 ± 0.02^d	0.96 ± 0.01^a	1.02 ± 0.01^b
		39	–	–	1.07 ± 0.01^e	0.88 ± 0.10^a	1.01 ± 0.01^b
H4K12-ace	0		1.00 ± 0.01^a	1.12 ± 0.02^a	1.49 ± 0.02^a	–	–
	1	37	1.12 ± 0.02^b	1.29 ± 0.01^b	1.66 ± 0.02^b	–	–
		39	–	–	1.27 ± 0.01^c	1.00 ± 0.01^a	1.08 ± 0.01^a
	2	37	–	–	1.19 ± 0.01^d	0.95 ± 0.01^b	1.01 ± 0.01^b
		39	–	–	1.03 ± 0.01^e	0.94 ± 0.01^b	0.97 ± 0.00^c
H4K16-ace	0		1.00 ± 0.01^a	1.06 ± 0.01^a	1.28 ± 0.01^a	–	–
	1	37	1.00 ± 0.02^a	1.11 ± 0.01^b	1.42 ± 0.01^b	–	–
		39	–	–	1.13 ± 0.01^c	0.99 ± 0.01^a	1.03 ± 0.01^a
	2	37	–	–	1.06 ± 0.01^d	0.99 ± 0.01^a	1.01 ± 0.01^b
		39	–	–	1.03 ± 0.01^e	0.97 ± 0.01^a	0.93 ± 0.01^b
H3K9-met	0	–	1.00 ± 0.01^a	1.02 ± 0.01^a	1.22 ± 0.01^a	–	–
	1	37	1.01 ± 0.01^a	1.22 ± 0.01^b	1.31 ± 0.01^b	–	–
		39	–	–	1.10 ± 0.01^c	0.97 ± 0.01^a	1.05 ± 0.01^a
	2	37	–	–	1.05 ± 0.01^d	0.94 ± 0.01^ab	1.03 ± 0.01^b
		39	–	–	0.98 ± 0.01^e	0.92 ± 0.01^b	0.97 ± 0.00^c
H3K4-met	0	–	1.00 ± 0.00^a	1.16 ± 0.01^a	1.33 ± 0.01^a	–	–
	1	37	1.08 ± 0.01^b	1.19 ± 0.01^a	1.41 ± 0.01^b	–	–
		39	–	–	1.25 ± 0.01^c	1.04 ± 0.01^a	1.14 ± 0.01^a
	2	37	–	–	1.19 ± 0.01^d	0.97 ± 0.01^b	1.12 ± 0.01^b
		39	–	–	0.99 ± 0.01^e	0.98 ± 0.02^b	0.96 ± 0.01^c
H3S10-pho	0	–	1.00 ± 0.01^a	1.20 ± 0.02^a	1.51 ± 0.02^a	–	–
	1	37	1.11 ± 0.02^b	1.33 ± 0.02^b	1.89 ± 0.02^b	–	–
		39	–	–	1.33 ± 0.02^c	0.99 ± 0.01^a	1.15 ± 0.02^a
	2	37	–	–	1.16 ± 0.02^d	0.95 ± 0.01^b	1.05 ± 0.02^b
		39	–	–	0.97 ± 0.01^e	0.88 ± 0.01^c	0.93 ± 0.01^c

^*Each treatment was repeated 3 times with each replicate containing 20–30 oocytes from prepubertal ovaries. For each histone site, the average relative fluorescence of the NSN oocytes before OH (0 h) was set to 1, and the averages of oocytes from other treatments within the same histone site were expressed relative to this value.

^a–eValues without a common letter in their superscripts differ (P < 0.05) in the same column within histone sites.

Table 2. Relative levels of acetylation (ace), dimethylation (met) or phosphorylation (pho) in different histone sites in oocytes with various chromatin configurations after OH at different temperatures for different times*.

Table 2. Relative levels of acetylation (ace), dimethylation (met) or phosphorylation (pho) in different histone sites in oocytes with various chromatin configurations after OH at different temperatures for different times^*.