Figure 3. Experimental cytoplasmic reduction to probe the role of cell size in nucleus-size setting.

(A) Experimental design. (B) Examples of control and cytoplasm-reduced embryos fixed and labelled at 4-cell stage. (C) Analysis of nuclear and cell volume in the resulting 4-cell embryos. Each point represents one cell. Red manipulated, white controls. Note the tight relationship between cell and nuclear size. R² = 0.67. Data from 82 nuclei over 4 replicates.