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. 2016 Jun 20;6:28040. doi: 10.1038/srep28040

Figure 5. Evidence that PKC, but not nuclear import, contributes to nucleus size-setting.

Figure 5

(A) NLS::GFP FRAP in 2-cell, 4-cell, and 8-cell stage embryos (n = 10, 9, 9 respectively from 3 replicates). Note there is no difference in the rate of recovery between developmental stages. (B) Live imaging of CAAX::GFP and H2B::RFP expressing embryos after interphase addition of PMA or chelyrethrine. (Chel, n = 20, PMA, n = 14 from 2 replicates) (C) Analysis of the effect of PMA or chel upon nucleus size following NER. A cartoon is shown of the experimental design, and images show typical examples of each treatment. Note that the treatments cause reciprocal changes in nucleus size. A minimum 37 measurements per experimental group from three replicates. All groups are significantly different to each other within a given developmental stage (P < 0.01 ANOVA for each comparison, actual values stated in main text).