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. 2016 Jun 20;6:28370. doi: 10.1038/srep28370

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Copyright © 2016, Macmillan Publishers Limited

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Colon tissue was collected to determine the NLRP3 positive signal by immunohistochemistry (A). Protein levels of NLRP3, ASC, caspase-1 in THP-1 cells were determined by Western blotting (B). The mRNA level of NLRP3 was determined by RT-PCR (C). The values presented are the mean ± S.E.M (n = 10 in each group).Number sign (#) indicates P < 0.01 vs. control group. Single asterisk (*) indicates P < 0.05, anddouble asterisks (**) indicate P < 0.01 vs. LPS group.