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. 2016 Jun 20;6:27864. doi: 10.1038/srep27864

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Copyright © 2016, Macmillan Publishers Limited

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Monocyte derived macrophages were infected with HIV in the presence/absence of cocaine for 10 days and apoptosis was measured by using FITC Annexin V Apoptosis Detection Kit I and the results were analyzed by Flow-cytometer. We have observed significant early apoptosis in HIV only infected macrophages (c) as well as in HIV infected macrophages grown in the presence of cocaine (d) when compared to the control cells (a). We did not see significant apoptosis in cocaine only exposed cells (b) when compared to the control cells. Although there is an increase but there is no statistically significantly increased early apoptosis in HIV infected cells grown in the presence of cocaine (d) in comparison with the HIV alone infected cells (c). No significant late apoptosis was observed in any of the test group cells when compared to the control cells (e). Figure 4 is the representative figure of three independent biological experiments and the values obtained were averaged. (**p ≤ 0.01; NS-Not Significant).