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. 2016 Jun 20;6:28343. doi: 10.1038/srep28343

Figure 3. Morphology changes of bESCs during long-term cultivation and karyotype analysis.

Figure 3

(A,E) ICM outgrowth appeared after 6 to 7 days of cultivation. Bar = 200 μm. (B,F) Domed colony similar to mouse ESCs at 3 passages was observed on MEFs feeder layer. Bar = 100 μm. (C) Morphology of control bESC colonies at passage 10 showing flat-shaped colonies similar to human ESCs. Bar = 200 μm. (D) Morphology of control bESC colonies at 15 passages formed cystic structure. Bar = 500 μm. (G) Morphology of CDX2-KD bESC colonies at 15 passages showing flat-shaped colonies similar to human ESCs. Bar = 200 μm. (H) CDX2-KD bESCs at 30 passages kept flat-shaped morphology without forming cystic structure. Bar = 500 μm. (I) Control bESCs at 17 passages showed normal karyotype. Bar = 5 μm. (J) CDX2-KD bESCs at 33 passages showed normal karyotype. Bar = 5 μm.