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. 2016 Jun 20;6:28343. doi: 10.1038/srep28343

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Copyright © 2016, Macmillan Publishers Limited

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Alkaline phosphatase staining of CDX2-KD bESCs (A), control bESCs at 8 passages (B) and 18 (C) passages. Bar = 200 μm. Compacted EBs derived from CDX2-KD bESCs at 34 passages (A′) and P8-bESCs (B′). GFP expression in CDX2-KD EBs was shown in inserted picture. Cystic embryoid bodies were derived from bESCs derived from P18-bESCs (C′). Bar = 500 μm. Express markers of three germ layers and trophoblast in these EBs. Ectodermal markers: NES, TUBB3 and KRT-8 (D). Mesodermal markers: T, BMP4 and MSX1 (E). Endodermal markers: GATA4, HNF4 and AFP (F). Trophoblast markers: ELF5, HAND1 and MASH2 (G). GAPDH was used as a loading control.