Table I. Primers used for quantitative reverse transcriptase-polymerase chain reactionq (qRT-PCR).
Primer | Gene name | Primer sequence |
---|---|---|
COX2_F: | Cyclo-oxygenase 2 | 5′-CCT TCCTCCTGTGCCTGATG-3′, |
COX2_R: | 5′-ACAATCTCATTTGAATCAGGAAGCT-3′ | |
COX2-6FAM | 5′-TGCCCGACTCCCTTGGGTGTCA-MGBNFQ | |
SPARC_R: | Secreted protein, acidic, cysteine-rich | 5′-TCTTCCCTGTACACTGGCAGTTC-3′ |
SPARC_F: | 5′-AGCTCGGTGTGGGAGAGGTA-3′ | |
SPARC-6FAM | 5′-CAGCTGGACCAGCACCCATTGACA-MGBNFQ | |
HIF1A_F: | Hypoxia inducible factor 1A | 5′-CTCATCCAAGAAGCCCTAACGTGTT-3′ |
HIF1A_R: | 5′-GCTTTCTCTGAGCATTCTGCAAAGC-3′ | |
HIF1A-6FAM | 5′-CCTCAGGAACTGTAGTTCTTTGACTCAAAGCGACA-MGBNFQ | |
CXCR4: | Chemokine (C-X-C motif) receptor 4 | Hs00607978_s1 (Applied Bioscience) |
PAX5C | Paired box 5 C-terminus | Hs00277134_m1 (Applied Bioscience) |
FOS_F: | FBJ murine osteosarcoma viral oncogene homolog | 5′-CGAGCCCTTTGTATGACTTCCT-3′ |
FOS_R: | 5′-GTCCATGTCTGGCACGGA G-3′ | |
FOS-6FAM | 5′-CCCAGCATCATCCAGGCCCAGTCA-MGBNFQ | |
KRAS_F: | Kristen rat sarcoma viral oncogene homolog | 5′-TTCCTACAGGAAGCA AGT AG-3′ |
KRAS_R: | 5′-CACAAAGAA AGCCCTCCCCA-3′ | |
KRAS-6FAM | 5′-TTGATGGAGAAACCTGTCTCTTGGCA-MGBNFQ | |
β-Actin_F: | 5′-GATGGCCACGGCTGCTT-3′ | |
β-Actin _R: | 5′-ACCGCTCATTGCCAATGG-3′ | |
β-Actin -6FAM | 5′-ACCACCACGGCCGAGCGGCA-MGMNFQ |
F: Forward primer; R: reverse primer; 6FAM: 6-carboxyfluorescin; MGBNFQ: molecular-groove binding non-fluorescence quencher.