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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Anticancer Res. 2015 Oct;35(10):5219–5223.

Table I. Primers used for quantitative reverse transcriptase-polymerase chain reactionq (qRT-PCR).

Primer Gene name Primer sequence
COX2_F: Cyclo-oxygenase 2 5′-CCT TCCTCCTGTGCCTGATG-3′,
COX2_R: 5′-ACAATCTCATTTGAATCAGGAAGCT-3′
COX2-6FAM 5′-TGCCCGACTCCCTTGGGTGTCA-MGBNFQ
SPARC_R: Secreted protein, acidic, cysteine-rich 5′-TCTTCCCTGTACACTGGCAGTTC-3′
SPARC_F: 5′-AGCTCGGTGTGGGAGAGGTA-3′
SPARC-6FAM 5′-CAGCTGGACCAGCACCCATTGACA-MGBNFQ
HIF1A_F: Hypoxia inducible factor 1A 5′-CTCATCCAAGAAGCCCTAACGTGTT-3′
HIF1A_R: 5′-GCTTTCTCTGAGCATTCTGCAAAGC-3′
HIF1A-6FAM 5′-CCTCAGGAACTGTAGTTCTTTGACTCAAAGCGACA-MGBNFQ
CXCR4: Chemokine (C-X-C motif) receptor 4 Hs00607978_s1 (Applied Bioscience)
PAX5C Paired box 5 C-terminus Hs00277134_m1 (Applied Bioscience)
FOS_F: FBJ murine osteosarcoma viral oncogene homolog 5′-CGAGCCCTTTGTATGACTTCCT-3′
FOS_R: 5′-GTCCATGTCTGGCACGGA G-3′
FOS-6FAM 5′-CCCAGCATCATCCAGGCCCAGTCA-MGBNFQ
KRAS_F: Kristen rat sarcoma viral oncogene homolog 5′-TTCCTACAGGAAGCA AGT AG-3′
KRAS_R: 5′-CACAAAGAA AGCCCTCCCCA-3′
KRAS-6FAM 5′-TTGATGGAGAAACCTGTCTCTTGGCA-MGBNFQ
β-Actin_F: 5′-GATGGCCACGGCTGCTT-3′
β-Actin _R: 5′-ACCGCTCATTGCCAATGG-3′
β-Actin -6FAM 5′-ACCACCACGGCCGAGCGGCA-MGMNFQ

F: Forward primer; R: reverse primer; 6FAM: 6-carboxyfluorescin; MGBNFQ: molecular-groove binding non-fluorescence quencher.